This article has been cited by other articles in PMC. Este obligatoriu sa ne oferiti consimtamantul inainte folosirea acestora pe site-ul nostru. Previously, subepithelial TCs were also found in the parotid gland; however, they were not tested for the expression of CD34 [ 24 ]. Histological evaluations used 3 μm-thick sections stained with hematoxylin and eosin. Poti sa refuzi acestea daca doresti. Sections were counterstained with hematoxylin and rinsed with deionized water.
Keywords: lymphatic endothelial cell, mesenchymal cell, intestinal stem niche, lamina propria, pericryptal fibroblasts 1.
Introduction Telocytes is the name given to interstitial cells with small cellular bodies, bearing prolongations telopodes that are extremely thin, long and moniliform [ 1 ]. It has been emphasised that the telopodial emergence and the size of the cell body and telopodes should differentiate TCs from various types of fibroblasts [ 1 ]. In TCs, cell prolongations directly emerge from the cell body the telopodial emergence and should be differentiated from hybrid morphologies or from a thick emergence of processes from the cell body, followed by a gradual thinning [ 1 ].
It has repeatedly been indicated that telopodes alone are not enough to distinguish TCs [ 12 ].
On two-dimensional cuts, the morphological appearance of TCs could be caused by tangential cuts of endothelial tubes. In tissues where TCs were previously identified using transmission electron microscopy TEMthree-dimensional ultrastructural studies demonstrated that TCs do not exist and, in fact, in single plane, the thin, flat cell body of pancake-like cells might occasionally be misinterpreted as telopodes [ 3 ].
The TCs identification by immunohistochemistry is still uncertain.
Теперь ты знаешь, что этот страх беспочвенен, что он искусственно внушен. Я, Ярлан Зей, вдохнувший его в тебя, теперь освобождаю тебя от его оков.
Additionally, TCs do not appear in the accepted Terminologia Histologica, and considerable artifactual results that have been used to support TCs as a novel cell type, are extensively documented [ 4567 ]. Diaz-Flores et al.
A different protocol for isolating normal and diseased LECs by multi-parameter fluorescence-activated cell sorting rejected the use of LYVE-1 [ 17 ] because that marker was found strongly expressed in the initial lymphatics but its expression was either very weak, or absent, in the lymphatic collecting vessels [ 18 ].
Nevertheless, Diaz-Flores et al.
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A recently published paper in Nature by Shoshkes-Carmel et al. Similar to Wnt5b, Wnts play key roles in vascular morphogenesis, vessels sprouting and elongation, but also in the development of lymphatic capillaries [ 202122 ].
Moreover, a single figure depicts such a SETC in transmission electron microscopy with such a small prolongation that can hardly be taken as a telopode [ 19 ]. Previously, subepithelial TCs were also found in the parotid gland; however, they were not tested for the expression of CD34 [ 24 ].
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This is a serious problem, as, in two-dimensional tangential cuts, LECs could appear as TC-like cells [ 26 ], hence the possibility of easily challenging the immunohistochemistry proofs.
We therefore intended to perform an immunohistochemical study using CD34 and podoplanin on human gastric wall samples, to critically review the results and document the publications identifying SETCs in the gut.
Material and Methods The immunohistochemical study was performed retrospectively on archived paraffin-embedded samples of human stomach ten cases. The ages of donor patients ranged from 56 to 62 years. Acest site foloseste cookie-uri pentru a-ti imbunatati experienta.
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